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pCEP4-FLAG-Tapasin-TAPBPR 22-35
(Plasmid #153478)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153478 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10381
  • Total vector size (bp) 11815
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TAPBP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1434
  • Mutation
    Tapasin a.a.10-20 replaced with TAPBPR a.a. 22-35
  • GenBank ID
    NP_003181
  • Entrez Gene
    TAPBP (a.k.a. NGS17, TAPA, TPN, TPSN)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV_fwd
  • 3′ sequencing primer EBV_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-FLAG-Tapasin-TAPBPR 22-35 was a gift from Erik Procko (Addgene plasmid # 153478 ; http://n2t.net/addgene:153478 ; RRID:Addgene_153478)

  • For your References section:

    TAPBPR promotes antigen loading on MHC-I molecules using a peptide trap. McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, Procko E, Sgourakis NG. Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6. 10.1038/s41467-021-23225-6 PubMed 34039964
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