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PurposeCROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffold
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153457 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCROP-seq
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Vector typeMammalian Expression, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsNone
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesgRNA empty backbone
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gRNA/shRNA sequenceFiler
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe CROP-sgRNA-MS2 lentiviral backbone was synthesized by VectorBuilder by adapting the CROP-seq vector (Datlinger et al. 2017) with the following modifications: 1) the sgRNA scaffold sequence contains two MS2 loops that allow recruitment of MS2-p65-HSF1 in SAM ESCs; and 2) a fluorescent mCherry marker was included downstream of the EF1α promoter and linked through T2A to a puromycin resistance cassette, allowing assessment of the multiplicity-of-infection (MOI) by FACS and antibiotic selection of the cells
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CROP-sgRNA-MS2 was a gift from Wolf Reik (Addgene plasmid # 153457 ; http://n2t.net/addgene:153457 ; RRID:Addgene_153457) -
For your References section:
A Single-Cell Transcriptomics CRISPR-Activation Screen Identifies Epigenetic Regulators of the Zygotic Genome Activation Program. Alda-Catalinas C, Bredikhin D, Hernando-Herraez I, Santos F, Kubinyecz O, Eckersley-Maslin MA, Stegle O, Reik W. Cell Syst. 2020 Jul 22;11(1):25-41.e9. doi: 10.1016/j.cels.2020.06.004. Epub 2020 Jul 6. 10.1016/j.cels.2020.06.004 PubMed 32634384