Skip to main content
Addgene

pET28a-Coh-ddFLN4-ELP-HIS-ybbr
(Plasmid #153446)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153446 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5219
  • Total vector size (bp) 6599
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Rc.CohE
  • Alt name
    Ruminococcus champanellensis cohesin E
  • Species
    Ruminococcus champanellensis
  • Insert Size (bp)
    1380
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • ddFLN4 fingerprint domain (C terminal on insert)
    • 3xELP linker (C terminal on insert)
    • 6xHis (C terminal on insert)
    • ybbr tag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer T7 forward primer
  • 3′ sequencing primer T7 reverse primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-Coh-ddFLN4-ELP-HIS-ybbr was a gift from Michael Nash (Addgene plasmid # 153446 ; http://n2t.net/addgene:153446 ; RRID:Addgene_153446)

  • For your References section:

    High force catch bond mechanism of bacterial adhesion in the human gut. Liu Z, Liu H, Vera AM, Bernardi RC, Tinnefeld P, Nash MA. Nat Commun. 2020 Aug 28;11(1):4321. doi: 10.1038/s41467-020-18063-x. 10.1038/s41467-020-18063-x PubMed 32859904
Related items: