pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)
(Plasmid
#153442)
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PurposeE. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153442 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5219
- Total vector size (bp) 7076
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRc.XDocB
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Alt nameRuminococcus champanellensis XModule-dockerin B
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SpeciesRuminococcus champanellensis
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Insert Size (bp)1857
- Promoter T7 promoter
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Tags
/ Fusion Proteins
- ybbr tag (N terminal on insert)
- 3xELP linker (N terminal on insert)
- ddFLN4 fingerprint domain (N terminal on insert)
- 6xHis (N terminal on insert)
- Titin I27 (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 forward primer
- 3′ sequencing primer T7 reverse primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.01.21.913590v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type) was a gift from Michael Nash (Addgene plasmid # 153442 ; http://n2t.net/addgene:153442 ; RRID:Addgene_153442) -
For your References section:
High force catch bond mechanism of bacterial adhesion in the human gut. Liu Z, Liu H, Vera AM, Bernardi RC, Tinnefeld P, Nash MA. Nat Commun. 2020 Aug 28;11(1):4321. doi: 10.1038/s41467-020-18063-x. 10.1038/s41467-020-18063-x PubMed 32859904
Map uploaded by the depositor.
