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pRepair-mTq2-CTNNB1
(Plasmid #153433)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153433 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS (+)
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 2965
  • Total vector size (bp) 5282
  • Vector type
    CRISPR ; HDR donor template

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CTNNB1 homology arms and mTurquoise2 coding sequence
  • Alt name
    CTNNB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2317
  • GenBank ID
  • Entrez Gene
    CTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
  • Tag / Fusion Protein
    • mTurquoise2 (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mTq2 was cloned from pmTurquoise2-C1 (Dorus Gadella, Plasmid #60560)
Homology arms were cloned from genomic HEK293 DNA. Please visit https://www.biorxiv.org/content/10.1101/2020.05.28.120543v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRepair-mTq2-CTNNB1 was a gift from Renee van Amerongen (Addgene plasmid # 153433 ; http://n2t.net/addgene:153433 ; RRID:Addgene_153433)

  • For your References section:

    Quantitative live-cell imaging and computational modeling shed new light on endogenous WNT/CTNNB1 signaling dynamics. de Man SM, Zwanenburg G, van der Wal T, Hink MA, van Amerongen R. Elife. 2021 Jun 30;10. pii: 66440. doi: 10.7554/eLife.66440. 10.7554/eLife.66440 PubMed 34190040
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