pRepair-mScI-CTNNB1
(Plasmid #153431)

• Purpose: Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i
• Depositing Lab: Renee van Amerongen
• Publication: de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi: 10.7554/eLife.66440.

Backbone

• Vector backbone: pBluescript KS (+)
• Backbone manufacturer: stratagene
• Backbone size w/o insert (bp): 2965
• Total vector size (bp): 5261
• Vector type: CRISPR ; HDR donor template

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CTNNB1 homology arms and mScI coding sequence
• Alt name: CTNNB1
• Species: H. sapiens (human)
• Insert Size (bp): 2296
• GenBank ID:
• Entrez Gene: CTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
• Tag / Fusion Protein:
  • mScarlet-i (N terminal on insert)

Resource Information

• Supplemental Documents:
  • pRepair-mScI-CTNNB1.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mScI was cloned from pmScarlet-i_C1 (Dorus Gadella, Plasmid #85044)
Homology arms were cloned from genomic HEK293 DNA. Please visit https://www.biorxiv.org/content/10.1101/2020.05.28.120543v1 for bioRxiv preprint.