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Addgene

pMIII-Neo-LO
(Plasmid #153428)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153428 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIII-Neo
  • Backbone manufacturer
    Maki Nakayama
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LSSmOrange
  • Species
    Other

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer 5pMIG
  • 3′ sequencing primer 3pMIG3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Second gene is neomycin-resistant gene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIII-Neo-LO was a gift from Maki Nakayama (Addgene plasmid # 153428 ; http://n2t.net/addgene:153428 ; RRID:Addgene_153428)

  • For your References section:

    Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation Reporter-Based Detection System. Mann SE, Zhou Z, Landry LG, Anderson AM, Alkanani AK, Fischer J, Peakman M, Mallone R, Campbell K, Michels AW, Nakayama M. Front Immunol. 2020 Apr 9;11:633. doi: 10.3389/fimmu.2020.00633. eCollection 2020. 10.3389/fimmu.2020.00633 PubMed 32328071
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