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Addgene

CaP2AIII_pK18
(Plasmid #153421)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153421 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKF18k02
  • Backbone manufacturer
    TaKaRa
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    murine TCR-alpha constant region (TRAC*02)-P2A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    525

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SacI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CaP2AIII_pK18 was a gift from Maki Nakayama (Addgene plasmid # 153421 ; http://n2t.net/addgene:153421 ; RRID:Addgene_153421)

  • For your References section:

    Human islet T cells are highly reactive to preproinsulin in type 1 diabetes. Anderson AM, Landry LG, Alkanani AA, Pyle L, Powers AC, Atkinson MA, Mathews CE, Roep BO, Michels AW, Nakayama M. Proc Natl Acad Sci U S A. 2021 Oct 12;118(41). pii: 2107208118. doi: 10.1073/pnas.2107208118. 10.1073/pnas.2107208118 PubMed 34611019
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