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Addgene

MBC2-PuroIII
(Plasmid #153420)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153420 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMI-Puro
  • Backbone manufacturer
    Maki Nakayama
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
  • Species
    M. musculus (mouse)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Second gene is puromycin-resistant gene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MBC2-PuroIII was a gift from Maki Nakayama (Addgene plasmid # 153420 ; http://n2t.net/addgene:153420 ; RRID:Addgene_153420)

  • For your References section:

    Human islet T cells are highly reactive to preproinsulin in type 1 diabetes. Anderson AM, Landry LG, Alkanani AA, Pyle L, Powers AC, Atkinson MA, Mathews CE, Roep BO, Michels AW, Nakayama M. Proc Natl Acad Sci U S A. 2021 Oct 12;118(41). pii: 2107208118. doi: 10.1073/pnas.2107208118. 10.1073/pnas.2107208118 PubMed 34611019
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