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CD3-2A_pMI-LO
(Plasmid #153418)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153418 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMI-LO
  • Backbone manufacturer
    Maki Nakayama
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    murine CD3 delta-gamma-epsilon-zeta subunits
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    711
  • Entrez Gene
    Cd3d (a.k.a. T3d)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Second gene is LSSmOrange

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CD3-2A_pMI-LO was a gift from Maki Nakayama (Addgene plasmid # 153418 ; http://n2t.net/addgene:153418 ; RRID:Addgene_153418)

  • For your References section:

    Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation Reporter-Based Detection System. Mann SE, Zhou Z, Landry LG, Anderson AM, Alkanani AK, Fischer J, Peakman M, Mallone R, Campbell K, Michels AW, Nakayama M. Front Immunol. 2020 Apr 9;11:633. doi: 10.3389/fimmu.2020.00633. eCollection 2020. 10.3389/fimmu.2020.00633 PubMed 32328071
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