Skip to main content
Addgene

pSKBAD2
(Plasmid #15335)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15335 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBAD
  • Backbone manufacturer
    Invitrogen but modified
  • Backbone size w/o insert (bp) 4771
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DnaE C-intein
  • Alt name
    Intein
  • Species
    Nostoc punctiforme
  • Insert Size (bp)
    117
  • Tag / Fusion Protein
    • GB1 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer pBAD-fw
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSKBAD2 was a gift from Hideo Iwai (Addgene plasmid # 15335 ; http://n2t.net/addgene:15335 ; RRID:Addgene_15335)

  • For your References section:

    Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. Iwai H, Zuger S, Jin J, Tam PH. FEBS Lett. 2006 Mar 20. 580(7):1853-8. 10.1016/j.febslet.2006.02.045 PubMed 16516207
Related items:
Commonly requested with: