FLAG-ZnF/HIT1
(Plasmid #15332)

• Depositing Labs: Joan Conaway, Ronald Conaway
• Publication: Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-70.

Backbone

• Vector backbone: pcDNA3.1/Hyg(+)
• Backbone size w/o insert (bp): 5601
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: zinc finger, HIT domain containing 1
• Alt name: ZnF/HIT1
• Species: H. sapiens (human)
• Insert Size (bp): 465
• Entrez Gene: ZNHIT1 (a.k.a. CG1I, ZNFN4A1)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry