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Addgene

pOpen_taq
(Plasmid #153315)

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Item Catalog # Description Quantity Price (USD)
Plasmid 153315 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pTTQ18
  • Backbone size w/o insert (bp) 4563
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Taq polymerase
  • Species
    Thermus aquaticus
  • Promoter Tac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (unknown if destroyed)
  • 5′ sequencing primer Tac promoter
  • 3′ sequencing primer M13_puc_F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid made by John Schloendorn at Gene And Cell Technologies. More information and protocols can be found on the FreeGene's webpage: https://stanford.freegenes.org/collections/covid-19/products/covid-19-diagnostic-toolkit-enzymes#description

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOpen_taq was a gift from Drew Endy (Addgene plasmid # 153315 ; http://n2t.net/addgene:153315 ; RRID:Addgene_153315)
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