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Addgene

FLAG ELL 500-621 pBacPAK8
(Plasmid #15331)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15331 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBacPAK8
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5538
  • Vector type
    Baculovirus construction

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eleven nineteen lysine rich in leukemia
  • Alt name
    ELL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    366
  • Mutation
    Amino acids 500-621 only
  • Entrez Gene
    ELL (a.k.a. C19orf17, ELL1, MEN, PPP1R68)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (destroyed during cloning)
  • 3′ cloning site Kpn1 (not destroyed)
  • 5′ sequencing primer Bac1
  • 3′ sequencing primer Bac2
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG ELL 500-621 pBacPAK8 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15331 ; http://n2t.net/addgene:15331 ; RRID:Addgene_15331)

  • For your References section:

    ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL. Kong SE, Banks CA, Shilatifard A, Conaway JW, Conaway RC. Proc Natl Acad Sci U S A. 2005 Jul 19. 102(29):10094-8. 10.1073/pnas.0503017102 PubMed 16006523
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