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pcDNA-IgKappa-myc-dL5- 2XG4S-GPI
(Plasmid #153308)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153308 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6405
  • Modifications to backbone
    NdeI site removed in CMV promoter
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IgKappa-myc-dL5-2XG4S-GPI
  • Alt name
    FAP-GPI
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1005
  • Promoter CMV
  • Tag / Fusion Protein
    • myc-dL5 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMVF
  • 3′ sequencing primer BGHR
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The GPI anchor amino acid sequence was designed from the findings in this paper.
Caras IW. 1991. An internally positioned signal can direct attachment of a glycophospholipid membrane anchor. J Cell Biol. 113(1):77-85.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-IgKappa-myc-dL5- 2XG4S-GPI was a gift from Marcel Bruchez (Addgene plasmid # 153308 ; http://n2t.net/addgene:153308 ; RRID:Addgene_153308)

  • For your References section:

    Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection in a Cyclophilin A-Dependent Manner. Zhong Z, Ning J, Boggs EA, Jang S, Wallace C, Telmer C, Bruchez MP, Ahn J, Engelman AN, Zhang P, Watkins SC, Ambrose Z. mBio. 2021 Mar 23;12(2). pii: mBio.03142-20. doi: 10.1128/mBio.03142-20. 10.1128/mBio.03142-20 PubMed 33758083
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