Skip to main content
Addgene

pAAV-GfaABC1D-GRAB_Ado1.0med
(Plasmid #153289)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153289 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 5121
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Adenosine sensor GRAB_Ado1.0med (medium affinity)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2256
  • Promoter GfaABC1D

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS identified a SNP in the WPRE. The depositor does not suspect this is of functional concern but requesting scientists should validate functionality before experimental use. Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.075564v1 for BioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-GfaABC1D-GRAB_Ado1.0med was a gift from Yulong Li (Addgene plasmid # 153289 ; http://n2t.net/addgene:153289 ; RRID:Addgene_153289)

  • For your References section:

    Neuronal activity-induced, equilibrative nucleoside transporter-dependent, somatodendritic adenosine release revealed by a GRAB sensor. Wu Z, Cui Y, Wang H, Wu H, Wan Y, Li B, Wang L, Pan S, Peng W, Dong A, Yuan Z, Jing M, Xu M, Luo M, Li Y. Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2212387120. doi: 10.1073/pnas.2212387120. Epub 2023 Mar 30. 10.1073/pnas.2212387120 PubMed 36996110
Related items:
Commonly requested with: