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PurposeExpress the adenosine sensor GRAB_Ado1.0 in astrocytes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153288 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 5121
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAdenosine sensor GRAB_Ado1.0
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SpeciesH. sapiens (human)
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Insert Size (bp)2259
- Promoter GfaABC1D
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.075564v1 for BioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-GfaABC1D-GRAB_Ado1.0 was a gift from Yulong Li (Addgene plasmid # 153288 ; http://n2t.net/addgene:153288 ; RRID:Addgene_153288) -
For your References section:
Regulation of sleep homeostasis mediator adenosine by basal forebrain glutamatergic neurons. Peng W, Wu Z, Song K, Zhang S, Li Y, Xu M. Science. 2020 Sep 4;369(6508). pii: 369/6508/eabb0556. doi: 10.1126/science.abb0556. 10.1126/science.abb0556 PubMed 32883833