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Addgene

pAAV-hsyn-GRAB_Ado1.0mut
(Plasmid #153287)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153287 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 4495
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2259
  • Promoter hSyn

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hsyn-GRAB_Ado1.0mut was a gift from Yulong Li (Addgene plasmid # 153287 ; http://n2t.net/addgene:153287 ; RRID:Addgene_153287)

  • For your References section:

    Regulation of sleep homeostasis mediator adenosine by basal forebrain glutamatergic neurons. Peng W, Wu Z, Song K, Zhang S, Li Y, Xu M. Science. 2020 Sep 4;369(6508). pii: 369/6508/eabb0556. doi: 10.1126/science.abb0556. 10.1126/science.abb0556 PubMed 32883833
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