pAAV-hsyn-GRAB_Ado1.0med
(Plasmid
#153286)
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PurposeExpress the medium-affinity adenosine sensor GRAB_Ado1.0med in neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153286 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 4495
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAdenosine sensor GRAB_Ado1.0med (medium affinity)
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SpeciesH. sapiens (human)
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Insert Size (bp)2256
- Promoter hSyn
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.075564v1 for BioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hsyn-GRAB_Ado1.0med was a gift from Yulong Li (Addgene plasmid # 153286 ; http://n2t.net/addgene:153286 ; RRID:Addgene_153286) -
For your References section:
Neuronal activity-induced, equilibrative nucleoside transporter-dependent, somatodendritic adenosine release revealed by a GRAB sensor. Wu Z, Cui Y, Wang H, Wu H, Wan Y, Li B, Wang L, Pan S, Peng W, Dong A, Yuan Z, Jing M, Xu M, Luo M, Li Y. Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2212387120. doi: 10.1073/pnas.2212387120. Epub 2023 Mar 30. 10.1073/pnas.2212387120 PubMed 36996110