Skip to main content
Addgene

1137B=Hsp70Bb-Cas9
(Plasmid #153284)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153284 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    AttB with mini-white
  • Total vector size (bp) 7300
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pHsp70Bb-SpCas9-T2A-eGFP-p10
  • Alt name
    Cas9 (Csn1) endonuclease from the Streptococcus pyogenes
  • Species
    Synthetic
  • Insert Size (bp)
    6132
  • Entrez Gene
    cas9 (a.k.a. B6D67_RS04280, ERS445054_00848)
  • Promoter Dmel Hsp70Bb
  • Tag / Fusion Protein
    • eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer taatgtatgctatacgaagttatgctagcggatccCTCCGTTGTATGTTGTAGCGTCTGT
  • 3′ sequencing primer TATAGTCCATtttgtttaaaCAGAGTTCTCTTCTTGTATTCAATAATTACTTCTTGGCAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1137B=Hsp70Bb-Cas9 was a gift from Omar Akbari (Addgene plasmid # 153284 ; http://n2t.net/addgene:153284 ; RRID:Addgene_153284)

  • For your References section:

    Temperature-Inducible Precision-Guided Sterile Insect Technique. Kandul NP, Liu J, Akbari OS. CRISPR J. 2021 Dec;4(6):822-835. doi: 10.1089/crispr.2021.0077. Epub 2021 Nov 3. 10.1089/crispr.2021.0077 PubMed 34735288
Related items: