pDGE672
(Plasmid #153238)

• Purpose: (Empty Backbone) Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
• Depositing Lab: Sylvestre Marillonnet
• Publication: Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197.

Backbone

• Vector backbone: pJOG292
• Backbone manufacturer: Ordon et al, 2017
• Backbone size (bp): 7000
• Vector type: Plant Expression, CRISPR
• Selectable markers: Hygromycin ; ccdB, , TagRFP-mNeptune (2XRFP), Bs3
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Resource Information

• Supplemental Documents:
  • pDGE672_hpt_2xRFP_Bs3__p35S GFP-Cas9_ccdB_(corrected2).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.