FLAG-EAF pBacPAK8
(Plasmid #15323)

• Depositing Labs: Joan Conaway, Ronald Conaway
• Publication: Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 102(29):10094-8.

Backbone

• Vector backbone: pBacPAK8
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5538
• Vector type: Baculovirus construction

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ELL Associated Factor
• Alt name: EAF
• Alt name: EAF1
• Species: H. sapiens (human)
• Insert Size (bp): 807
• Entrez Gene: EAF1
• Promoter: polyhedrin
• Tags / Fusion Proteins:
  • 6x His (N terminal on backbone)
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: Kpn1 (not destroyed)
• 5′ sequencing primer: Bac1
• 3′ sequencing primer: Bac2

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry