Skip to main content
Addgene

FLAG-SpEAF 60-251 pBacPAK8
(Plasmid #15321)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15321 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBacPAK8
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5538
  • Vector type
    Baculovirus construction

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EAF1
  • Alt name
    SpEAF
  • Alt name
    eaf1
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    600
  • Mutation
    N terminal deletion lacking first 59 amino acids of SpEAF
  • Entrez Gene
    eaf1 (a.k.a. SPCC1223.10c)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Kpn1 (not destroyed)
  • 5′ sequencing primer Bac1
  • 3′ sequencing primer Bac2
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-SpEAF 60-251 pBacPAK8 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15321 ; http://n2t.net/addgene:15321 ; RRID:Addgene_15321)

  • For your References section:

    Identification and Characterization of a Schizosaccharomyces pombe RNA Polymerase II Elongation Factor with Similarity to the Metazoan Transcription Factor ELL. Banks CA, Kong SE, Spahr H, Florens L, Martin-Brown S, Washburn MP, Conaway JW, Mushegian A, Conaway RC. J Biol Chem. 2007 Feb 23. 282(8):5761-9. 10.1074/jbc.M610393200 PubMed 17150956
Related items: