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Addgene

CARPID BASU-dCasRx
(Plasmid #153209)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153209 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EF1a-dCasRx-2A-EGFP (Addgene no. 109050)
  • Backbone manufacturer
    Patrick Hsu
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BASU-dCasRx
  • Alt name
    BASU RaPID biotin ligase fused to N-terminus of NLS-dRfxCas13d-NLS
  • Species
    H. sapiens (human)
  • Mutation
    R239A/H244A/R858A/H863A in RfxCas13d
  • Promoter EF-1a

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    BASU was subcloned from RaPID plasmid (Addgene no. 107250) into the backbone of an EF1a-dCasRx-2A-EGFP plasmid (Addgene no. 109050)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CARPID BASU-dCasRx was a gift from Jian Yan & Liang Zhang (Addgene plasmid # 153209 ; http://n2t.net/addgene:153209 ; RRID:Addgene_153209)

  • For your References section:

    CRISPR-assisted detection of RNA-protein interactions in living cells. Yi W, Li J, Zhu X, Wang X, Fan L, Sun W, Liao L, Zhang J, Li X, Ye J, Chen F, Taipale J, Chan KM, Zhang L, Yan J. Nat Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 10.1038/s41592-020-0866-0. 10.1038/s41592-020-0866-0 PubMed 32572232
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