SARS-CoV2 Nucleocapsid
(Plasmid #153201)

• Purpose: Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells
• Depositing Lab: Peter Klein
• Publication: Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;118(42). pii: 2113401118. doi: 10.1073/pnas.2113401118. Epub 2021 Sep 30.

Backbone

• Vector backbone: pCS2+MT
• Backbone manufacturer: Turner and Rupp
• Backbone size w/o insert (bp): 4352
• Total vector size (bp): 5565
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nucleocapsid from SARS-CoV-2
• Alt name: N (Severe acute respiratory syndrome coronavirus 2)
• Species: severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
• Insert Size (bp): 1260
• GenBank ID: MT385497.1
• Entrez Gene: N (a.k.a. GU280_gp10)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc tag (5X) (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Construct made by Xiaolei Liu/Klein Lab