MPC020: CamKII CMV_NovArch_citrine
(Plasmid
#153193)
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PurposeEncodes Novarch
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153193 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFCK
- Backbone size w/o insert (bp) 9239
- Total vector size (bp) 10994
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNovArch
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SpeciesSynthetic
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Insert Size (bp)1755
- Promoter CMV
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Tag
/ Fusion Protein
- citrine
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GACACCGACTCTAGAGCGCGGATCCACCATGGTAAGTATC
- 3′ sequencing primer TCTCGTAGCAGAACTTGTAGAATTCTTATTCATTCTCATAACAAAAG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MPC020: CamKII CMV_NovArch_citrine was a gift from Adam Cohen (Addgene plasmid # 153193 ; http://n2t.net/addgene:153193 ; RRID:Addgene_153193) -
For your References section:
Photoactivated voltage imaging in tissue with an archaerhodopsin-derived reporter. Chien MP, Brinks D, Testa-Silva G, Tian H, Phil Brooks F 3rd, Adam Y, Bloxham W, Gmeiner B, Kheifets S, Cohen AE. Sci Adv. 2021 May 5;7(19). pii: 7/19/eabe3216. doi: 10.1126/sciadv.abe3216. Print 2021 May. 10.1126/sciadv.abe3216 PubMed 33952514