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Addgene

MPC020: CamKII CMV_NovArch_citrine
(Plasmid #153193)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153193 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    FCK
  • Backbone size w/o insert (bp) 9239
  • Total vector size (bp) 10994
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NovArch
  • Species
    Synthetic
  • Insert Size (bp)
    1755
  • Promoter CMV
  • Tag / Fusion Protein
    • citrine

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GACACCGACTCTAGAGCGCGGATCCACCATGGTAAGTATC
  • 3′ sequencing primer TCTCGTAGCAGAACTTGTAGAATTCTTATTCATTCTCATAACAAAAG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MPC020: CamKII CMV_NovArch_citrine was a gift from Adam Cohen (Addgene plasmid # 153193 ; http://n2t.net/addgene:153193 ; RRID:Addgene_153193)
  • For your References section:

    Photoactivated voltage imaging in tissue with an archaerhodopsin-derived reporter. Chien MP, Brinks D, Testa-Silva G, Tian H, Phil Brooks F 3rd, Adam Y, Bloxham W, Gmeiner B, Kheifets S, Cohen AE. Sci Adv. 2021 May 5;7(19). pii: 7/19/eabe3216. doi: 10.1126/sciadv.abe3216. Print 2021 May. 10.1126/sciadv.abe3216 PubMed 33952514