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Addgene

10 x FHR-luc
(Plasmid #153161)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153161 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3
  • Backbone size w/o insert (bp) 4853
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    luciferase
  • Species
    Other
  • Insert Size (bp)
    127
  • Promoter minP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer RVPrimer3
  • 3′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    10 x FHR-luc was a gift from Stefan Koch (Addgene plasmid # 153161 ; http://n2t.net/addgene:153161 ; RRID:Addgene_153161)

  • For your References section:

    A uniform expression library for the exploration of FOX transcription factor biology. Moparthi L, Koch S. Differentiation. 2020 Sep - Oct;115:30-36. doi: 10.1016/j.diff.2020.08.002. Epub 2020 Aug 20. 10.1016/j.diff.2020.08.002 PubMed 32858261
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