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Addgene

FLAG-SpELL pNMT-TOPO
(Plasmid #15315)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNMT-TOPO (modified)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6066
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eleven nineteen lysine rich in leukemia
  • Alt name
    SpELL
  • Alt name
    ell1
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    1602
  • Entrez Gene
    ell1
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Kpn1 (not destroyed)
  • 5′ sequencing primer NMT forward
  • 3′ sequencing primer URA4 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-SpELL pNMT-TOPO was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15315 ; http://n2t.net/addgene:15315 ; RRID:Addgene_15315)

  • For your References section:

    Identification and Characterization of a Schizosaccharomyces pombe RNA Polymerase II Elongation Factor with Similarity to the Metazoan Transcription Factor ELL. Banks CA, Kong SE, Spahr H, Florens L, Martin-Brown S, Washburn MP, Conaway JW, Mushegian A, Conaway RC. J Biol Chem. 2007 Feb 23. 282(8):5761-9. 10.1074/jbc.M610393200 PubMed 17150956
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