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PurposeExpression of Flag tagged human FOXP1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153145 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+ N-flag
- Backbone size w/o insert (bp) 4131
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman FOXP1
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SpeciesH. sapiens (human)
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Insert Size (bp)2032
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Entrez GeneFOXP1 (a.k.a. 12CC4, HSPC215, MFH, QRF1, hFKH1B)
- Promoter CMV IE94
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Second amino acid methionine is removed
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Flag-FOXP1 was a gift from Stefan Koch (Addgene plasmid # 153145 ; http://n2t.net/addgene:153145 ; RRID:Addgene_153145) -
For your References section:
A uniform expression library for the exploration of FOX transcription factor biology. Moparthi L, Koch S. Differentiation. 2020 Sep - Oct;115:30-36. doi: 10.1016/j.diff.2020.08.002. Epub 2020 Aug 20. 10.1016/j.diff.2020.08.002 PubMed 32858261
