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Addgene

Flag-FOXO3
(Plasmid #153142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+ N-flag
  • Backbone size w/o insert (bp) 4131
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human FOXO3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2026
  • Entrez Gene
    FOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
  • Promoter CMV IE94
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-FOXO3 was a gift from Stefan Koch (Addgene plasmid # 153142 ; http://n2t.net/addgene:153142 ; RRID:Addgene_153142)

  • For your References section:

    A uniform expression library for the exploration of FOX transcription factor biology. Moparthi L, Koch S. Differentiation. 2020 Sep - Oct;115:30-36. doi: 10.1016/j.diff.2020.08.002. Epub 2020 Aug 20. 10.1016/j.diff.2020.08.002 PubMed 32858261
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