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Addgene

pCDNA3 Flag MKK4(ala)
(Plasmid #15314)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15314 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MKK4(ala)
  • Alt name
    MKK4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1151
  • Mutation
    Ser-220Ala Thr-224Ala
  • Entrez Gene
    MAP2K4 (a.k.a. JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 Flag MKK4(ala) was a gift from Roger Davis (Addgene plasmid # 15314 ; http://n2t.net/addgene:15314 ; RRID:Addgene_15314)
  • For your References section:

    Integration of MAP kinase signal transduction pathways at the serum response element. Whitmarsh AJ, Shore P, Sharrocks AD, Davis RJ. Science. 1995 Jul 21. 269(5222):403-7. 10.1126/science.7618106 PubMed 7618106