pCbeta EV (PKA catalytic subunit Cbeta)
(Plasmid #15311)

• Depositing Lab: Stanley McKnight
• Publication: Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7.

Backbone

• Vector backbone: Zem3 (pUC13 + MT prom + hGH polyA)
• Backbone manufacturer: Dr. Richard Palmiter
• Backbone size w/o insert (bp): 4100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PRKACB
• Alt name: cAMP-dependent protein kinase catalytic subunit beta
• Alt name: PKACB
• Species: M. musculus (mouse)
• Insert Size (bp): 1246
• Entrez Gene: Prkacb (a.k.a. CbPKA, Pkacb)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Zem3 is a pUC13 derivative that contains a mouse metallothionein-I promoter and the human growth hormone pol(A) region separated by a BglII restriction site. pCbeta EV was constructed by isolating the 1246bp SacI fragment of pMCbeta and inserting it into the BglII site of Zem3 after ligation of BamHI linkers onto the cDNA fragment.