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Borodina Yeast Transporter Libraries
(Pooled Libraries #153101, 153102, 153103, 153104, 153105, 153106)

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  • Purpose

    These yeast pooled CRISPR libraries target all 361 non-essential and conditionally essential transporter genes in S. cerevisiae. Plasmids contain both an sgRNA and corresponding 165 bp donor fragment, achieving high efficiency disruption of the target genes by introduction of frameshift mutations.

    Libraries are divided into five sub-groups targeting different groups of transporters.

    • Sctrans-PP-Grp1: ATP dependent transporters
    • Sctrans-PP-Grp2: Secondary transporters (SeTS-1 to SeTS-5)
    • Sctrans-PP-Grp3: Secondary transporters (SeTS-6 to SeTS-9)
    • Sctrans-PP-Grp4: Secondary transporters (SeTS-10 to SeTS-14)
    • Sctrans-PP-Grp5: Ion channel, unclassified, and other transporters
    • Sctrans-PP targets all 361 genes

  • Vector Backbone

    pMEL10 (Plasmid #107916) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 153102 Sctrans-PP-Grp1† 1 $325 Add to Cart
Pooled Library 153103 Sctrans-PP-Grp2† 1 $325 Add to Cart
Pooled Library 153104 Sctrans-PP-Grp3† 1 $325 Add to Cart
Pooled Library 153105 Sctrans-PP-Grp4† 1 $325 Add to Cart
Pooled Library 153106 Sctrans-PP-Grp5† 1 $325 Add to Cart
Pooled Library 153101 Sctrans-PP† 1 $380 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item. These libraries should be used with a yeast strain constitutively expressing SpCas9.

Library Details

Pooled Library Name Genes Targeted
153102 Sctrans-PP-Grp1 73
153103 Sctrans-PP-Grp2 79
153104 Sctrans-PP-Grp3 65
153105 Sctrans-PP-Grp4 84
153106 Sctrans-PP-Grp5 60
153101 Sctrans-PP 361
  • Species
    S. cerevisiae

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    25 ng/µL

Resource Information

Depositor Comments

The depositing lab's raw NGS data can be downloaded from the European Nucleotide Archive (ENA) — Project: PRJEB39414 (Link opens in a new window)

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Yeast Transporter Sctrans-PP-Grp1 Library was a gift from Irina Borodina (Addgene #153102)
    Yeast Transporter Sctrans-PP-Grp2 Library was a gift from Irina Borodina (Addgene #153103)
    Yeast Transporter Sctrans-PP-Grp3 Library was a gift from Irina Borodina (Addgene #153104)
    Yeast Transporter Sctrans-PP-Grp4 Library was a gift from Irina Borodina (Addgene #153105)
    Yeast Transporter Sctrans-PP-Grp5 Library was a gift from Irina Borodina (Addgene #153106)
    Yeast Transporter Sctrans-PP Library was a gift from Irina Borodina (Addgene #153107)

  • For your References section:

    Transportome-wide engineering of Saccharomyces cerevisiae. Wang G, Moller-Hansen I, Babaei M, D'Ambrosio V, Christensen HB, Darbani B, Jensen MK, Borodina I. Metab Eng. 2021 Jan 16. pii: S1096-7176(21)00015-X. doi: 10.1016/j.ymben.2021.01.007. PubMed 33465478
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