pAAV-syn-FLEX-synaptoPAC-minWPRE
(Plasmid #153100)

• Purpose: Cre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons
• Depositing Lab: Dietmar Schmitz
• Publication: Oldani et al J Neurochem. 2021 Feb;156(3):324-336. doi: 10.1111/jnc.15210. Epub 2020 Oct 22.

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Backbone

• Vector backbone: pAAV CW3SL-eGFP (Addgene 61463)
• Backbone size w/o insert (bp): 4136
• Total vector size (bp): 6926
• Modifications to backbone: CamKII promoter was replaced by synapsin promoter. EGFP removed, inserted FLEX cassette (Atasoy 2008, JNeurosci).
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Researchers should avoid repetitive freeze-thaw cycles when handling the DNA, as we found that this can lead to DNA degradation. Recommended growth strains are XL10gold.
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: bPAC(WT)
• Alt name: photoactivated adenylyl cyclase from Beggiatoa sp.
• Species: Beggiatoa sp.
• Insert Size (bp): 1055
• GenBank ID: GU461307
• Tags / Fusion Proteins:
  • myc tag (C terminal on insert)
  • His tag (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: Synaptophysin
• Alt name: syp1
• Species: R. norvegicus (rat)
• GenBank ID: 24804
• Entrez Gene: Syp (a.k.a. Syp1)
• Tag / Fusion Protein:
  • mScarlet (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pAAV-Syn-FLEX-SYNAPTOPAC_minWPRE.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry