pCalpha EV (PKA catalytic subunit Calpha)
(Plasmid #15310)

• Depositing Lab: Stanley McKnight
• Publication: Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7.

Backbone

• Vector backbone: Zem3 (pUC13 + MT prom + hGH polyA)
• Backbone manufacturer: Dr. Richard Palmiter
• Backbone size w/o insert (bp): 4100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PRKACA
• Alt name: cAMP-dependent protein kinase catalytic subunit alpha
• Species: M. musculus (mouse)
• Insert Size (bp): 1318
• Entrez Gene: Prkaca (a.k.a. PKCD, Pkaca)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Zem3 is a pUC13 derivative that contains a mouse metallothionein-I promoter and the human growth hormone pol(A) region separated by a BglII restriction site. pCalpha EV was constructed by isolating the 1318 base pair NaeI/PvuII fragment of pMCalpha and inserting it into the BglII site of Zem3 after ligation of BamHI linkers onto the cDNA insert.

The coding sequence can be cut out with NcoI and ApaI, which should release a 1152bp fragment.