pNM3732
(Plasmid
#153086)
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Purposemec-4p GFP-C1 positive control for RMCE integration
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153086 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLF3FShC
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Backbone manufacturerAddgene #153083
- Backbone size w/o insert (bp) 7855
- Total vector size (bp) 9648
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Modifications to backbonenone
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Vector typeWorm Expression ; RMCE
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemec-4p GFP-C1 tbb-2 3'
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SpeciesC. elegans (nematode), Synthetic
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Insert Size (bp)1829
- Promoter not applicable
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer not applicable (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe insert was added using a multi-fragment Golden Gate reaction with the mec-4 promoter and tbb-2 3' UTR coming from amplified genomic sequences and GFP-C1 from Addgene #91824.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNM3732 was a gift from Michael Nonet (Addgene plasmid # 153086 ; http://n2t.net/addgene:153086 ; RRID:Addgene_153086) -
For your References section:
Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange. Nonet ML. Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388. 10.1534/genetics.120.303388 PubMed 32513816