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Addgene

pNM3732
(Plasmid #153086)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153086 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLF3FShC
  • Backbone manufacturer
    Addgene #153083
  • Backbone size w/o insert (bp) 7855
  • Total vector size (bp) 9648
  • Modifications to backbone
    none
  • Vector type
    Worm Expression ; RMCE
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mec-4p GFP-C1 tbb-2 3'
  • Species
    C. elegans (nematode), Synthetic
  • Insert Size (bp)
    1829
  • Promoter not applicable

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The insert was added using a multi-fragment Golden Gate reaction with the mec-4 promoter and tbb-2 3' UTR coming from amplified genomic sequences and GFP-C1 from Addgene #91824.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNM3732 was a gift from Michael Nonet (Addgene plasmid # 153086 ; http://n2t.net/addgene:153086 ; RRID:Addgene_153086)
  • For your References section:

    Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange. Nonet ML. Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388. 10.1534/genetics.120.303388 PubMed 32513816