pLF3FShC
(Plasmid #153083)

• Purpose: (Empty Backbone) RMCE integration vector with self excising cassette
• Depositing Lab: Michael Nonet
• Publication: Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.

Backbone

• Vector backbone: pBluescript II SK (+)
• Backbone manufacturer: Stratagene Cloning Systems
• Modifications to backbone: 1) Removal of SapI site in pBluescript II SK (+), 2) Addition of a loxP site, MCS, FRT3 site, FRT site, hygromycin resistance gene, let-858 3' UTR, sqt-1(e1350) gene, hsp-16.41 promoter, Cre recombinase gene, and tbb-2 3' UTR, all via multi-insert Golden Gate Assembly 3) insertion of two SapI sites in MCS.
• Vector type: Worm Expression ; Recombination Mediated Cassette Exchange
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • pLF3FShC.gb
• A portion of this plasmid was derived from a plasmid made by: Some component parts were obtained from other plasmids. Specifically, the hygR gene, sqt-1(e1350) gene, hsp-16-41 promoter, let-858 and tbb-2 3' UTR were all PCR amplified from Addgene #91829 made by Daniel Dickinson in Bob Goldstein's lab
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry