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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 15308 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCaST-elav
- Backbone size w/o insert (bp) 13475
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVP16AD-ZIP+
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Alt nameVP16AD-EE12RR345L
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Insert Size (bp)500
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The heterodimerizing leucine zipper is based on the chicken B-Zip family member Vitellogenin Binding Protein. The EE12RR345L (Zip+) domain was generated from synthetic oligonucleotides with codon usage optimized for Drosophila expression.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCaST-elav-VP16AD was a gift from Benjamin White (Addgene plasmid # 15308 ; http://n2t.net/addgene:15308 ; RRID:Addgene_15308) -
For your References section:
Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression. Luan H, Peabody NC, Vinson CR, White BH. Neuron. 2006 Nov 9. 52(3):425-36. 10.1016/j.neuron.2006.08.028 PubMed 17088209
Map uploaded by the depositor.
