Skip to main content
Addgene

pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP
(Plasmid #153071)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153071 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLVX-EF1α-IRES-EGFP
  • Backbone manufacturer
    GENECOPOEIA
  • Backbone size w/o insert (bp) 7349
  • Total vector size (bp) 8933
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mutated ZC3H12D (D95N)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1584
  • Mutation
    Changed D95 to N, known to abrogate the RNase enzymatic activity
  • Entrez Gene
    ZC3H12D (a.k.a. C6orf95, MCPIP4, TFL, dJ281H8.1, p34)
  • Promoter EF1α

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original wild-type, unmutated vector was purchased from GENECOPOEIA (catalog number EX-Y4955-Lv165). Starting from this vector, amino acid D95 was mutated to N.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP was a gift from Silvia Monticelli (Addgene plasmid # 153071 ; http://n2t.net/addgene:153071 ; RRID:Addgene_153071)

  • For your References section:

    A molecular network regulating the proinflammatory phenotype of human memory T lymphocytes. Emming S, Bianchi N, Polletti S, Balestrieri C, Leoni C, Montagner S, Chirichella M, Delaleu N, Natoli G, Monticelli S. Nat Immunol. 2020 Apr;21(4):388-399. doi: 10.1038/s41590-020-0622-8. Epub 2020 Mar 16. 10.1038/s41590-020-0622-8 PubMed 32205878
Related items:
Commonly requested with: