LARS_pNIC-Bio3
(Plasmid
#153052)
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PurposeExpression construct for producing Avi tagged Leucyl-tRNA synthetase
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153052 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNIC-Bio3
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Backbone manufacturerStructural Genomics Consortium
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLARS
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SpeciesH. sapiens (human)
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MutationNot full length protein
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Entrez GeneLARS1 (a.k.a. HSPC192, ILFS1, LARS, LEURS, LEUS, LFIS, LRS, PIG44, RNTLS, cLRS, hr025Cl)
- Promoter T7
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Tags
/ Fusion Proteins
- His6-TEV (N terminal on backbone)
- Avi (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Backbone vector pNIC-Bio3, Genbank acc. no. JN792439. Please note that this plasmid may not encode full length LARS. Please see the plasmid sequences for additional information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LARS_pNIC-Bio3 was a gift from Susanne Gräslund (Addgene plasmid # 153052 ; http://n2t.net/addgene:153052 ; RRID:Addgene_153052)