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GARS_pNIC-Bio3
(Plasmid #153049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC-Bio3
  • Backbone manufacturer
    Structural Genomics Consortium
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GARS
  • Alt name
    EJ
  • Species
    H. sapiens (human)
  • Entrez Gene
    GARS1 (a.k.a. CMT2D, DSMAV, GARS, GlyRS, HMN5, HMN5A, HMND5, SMAD1, SMAJI)
  • Promoter T7
  • Tags / Fusion Proteins
    • His6-TEV (N terminal on backbone)
    • Avi (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone vector pNIC-Bio3, Genbank acc. no. JN792439

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GARS_pNIC-Bio3 was a gift from Susanne Gräslund (Addgene plasmid # 153049 ; http://n2t.net/addgene:153049 ; RRID:Addgene_153049)
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