pTF-lacZ-rfp
(Plasmid
#153037)
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PurposeCRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153037 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTF
- Total vector size (bp) 4274
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCRISPR array
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Insert Size (bp)3921
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTF-lacZ-rfp was a gift from Nigel Scrutton (Addgene plasmid # 153037 ; http://n2t.net/addgene:153037 ; RRID:Addgene_153037) -
For your References section:
A plasmid toolset for CRISPR-mediated genome editing and CRISPRi gene regulation in Escherichia coli. Jervis AJ, Hanko EKR, Dunstan MS, Robinson CJ, Takano E, Scrutton NS. Microb Biotechnol. 2021 Mar 12. doi: 10.1111/1751-7915.13780. 10.1111/1751-7915.13780 PubMed 33710766
