pSIMcpf1
(Plasmid
#153034)
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PurposeFor CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153034 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSIM18
- Total vector size (bp) 12415
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAscpf1
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SpeciesAcidaminococcus sp. BV3L6
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Insert Size (bp)3921
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSIMcpf1 was a gift from Nigel Scrutton (Addgene plasmid # 153034 ; http://n2t.net/addgene:153034 ; RRID:Addgene_153034) -
For your References section:
A plasmid toolset for CRISPR-mediated genome editing and CRISPRi gene regulation in Escherichia coli. Jervis AJ, Hanko EKR, Dunstan MS, Robinson CJ, Takano E, Scrutton NS. Microb Biotechnol. 2021 Mar 12. doi: 10.1111/1751-7915.13780. 10.1111/1751-7915.13780 PubMed 33710766
