Py2087ACR1
(Plasmid #153032)

• Purpose: Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).
• Depositing Lab: Peter Hegemann
• Publication: Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.e5. doi: 10.1016/j.cub.2020.09.056. Epub 2020 Oct 15.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 3986
• Total vector size (bp): 6278
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Py2087ACR1
• Species: Pyramimonas spec.
• Insert Size (bp): 1575
• GenBank ID: MT353681
• Promoter: CMV (+ enhancer)
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BshTI (not destroyed)
• 5′ sequencing primer: CMV-fwd: GCAAATGGGCGGTAGGCGT or EGFP-N1-fwd: GAGGTCTATATAAGCAGAGC
• 3′ sequencing primer: EGFP-C1-rev: AACCATTATAAGCTGCAATAAAC

Resource Information

• Supplemental Documents:
  • Py2087ACR1_mCherry.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry