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Addgene

PymeACR1
(Plasmid #153031)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153031 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 3986
  • Total vector size (bp) 6311
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PymeACR1
  • Species
    Pyramimonas melkonianii
  • Insert Size (bp)
    1608
  • GenBank ID
    MT353682
  • Promoter CMV (+ enhancer)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BshTI (not destroyed)
  • 5′ sequencing primer CMV-fwd: GCAAATGGGCGGTAGGCGT or EGFP-N1-fwd: GAGGTCTATATAAGCAGAGC
  • 3′ sequencing primer EGFP-C1-rev: AACCATTATAAGCTGCAATAAAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PymeACR1 was a gift from Peter Hegemann (Addgene plasmid # 153031 ; http://n2t.net/addgene:153031 ; RRID:Addgene_153031)

  • For your References section:

    Lateral Gene Transfer of Anion-Conducting Channelrhodopsins between Green Algae and Giant Viruses. Rozenberg A, Oppermann J, Wietek J, Fernandez Lahore RG, Sandaa RA, Bratbak G, Hegemann P, Beja O. Curr Biol. 2020 Dec 21;30(24):4910-4920.e5. doi: 10.1016/j.cub.2020.09.056. Epub 2020 Oct 15. 10.1016/j.cub.2020.09.056 PubMed 33065010
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