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Addgene

pJF143.J3
(Plasmid #153028)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153028 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SC101** vector
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    J3-mRFP1
  • Species
    Synthetic

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJF143.J3 was a gift from Jesse Zalatan (Addgene plasmid # 153028 ; http://n2t.net/addgene:153028 ; RRID:Addgene_153028)

  • For your References section:

    Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements. Fontana J, Dong C, Kiattisewee C, Chavali VP, Tickman BI, Carothers JM, Zalatan JG. Nat Commun. 2020 Apr 1;11(1):1618. doi: 10.1038/s41467-020-15454-y. 10.1038/s41467-020-15454-y PubMed 32238808
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