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Addgene

pCD565
(Plasmid #153027)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153027 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15A vector
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA
  • Species
    Evolved (dxCas9(3.7)), MS2 (MCP), E. coli (SoxS), synthetic (J306 scRNA)
  • Mutation
    SoxS has R93A and S101A mutations

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    dxCas9(3.7) was a gift from David Liu
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCD565 was a gift from Jesse Zalatan (Addgene plasmid # 153027 ; http://n2t.net/addgene:153027 ; RRID:Addgene_153027)

  • For your References section:

    Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements. Fontana J, Dong C, Kiattisewee C, Chavali VP, Tickman BI, Carothers JM, Zalatan JG. Nat Commun. 2020 Apr 1;11(1):1618. doi: 10.1038/s41467-020-15454-y. 10.1038/s41467-020-15454-y PubMed 32238808
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