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Addgene

OA-1136J
(Plasmid #153023)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153023 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET His6 MBP TEV LIC cloning vector (1M)
  • Backbone size w/o insert (bp) 6430
  • Total vector size (bp) 9328
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CasRx
  • Alt name
    NLS-RfxCas13d-NLS
  • Species
    Ruminococcus flavefaciens
  • Insert Size (bp)
    2898
  • Promoter T7
  • Tag / Fusion Protein
    • His-MBP-TEV (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OA-1136J was a gift from Omar Akbari (Addgene plasmid # 153023 ; http://n2t.net/addgene:153023 ; RRID:Addgene_153023)

  • For your References section:

    Development of a Rapid and Sensitive CasRx-Based Diagnostic Assay for SARS-CoV-2. Brogan DJ, Chaverra-Rodriguez D, Lin CP, Smidler AL, Yang T, Alcantara LM, Antoshechkin I, Liu J, Raban RR, Belda-Ferre P, Knight R, Komives EA, Akbari OS. ACS Sens. 2021 Nov 26;6(11):3957-3966. doi: 10.1021/acssensors.1c01088. Epub 2021 Oct 29. 10.1021/acssensors.1c01088 PubMed 34714054
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