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Addgene

pV2A-T
(Plasmid #153022)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153022 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVV.01
  • Backbone manufacturer
    Vito Valiante
  • Backbone size (bp) 1820
  • Modifications to backbone
    Insertion of a split YFP, single EcoRV cloning site, TetON promoter systhem, and a TrpC terminator
  • Vector type
    Bacterial Expression, Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pV2A-T was a gift from Vito Valiante (Addgene plasmid # 153022 ; http://n2t.net/addgene:153022 ; RRID:Addgene_153022)

  • For your References section:

    Facile assembly and fluorescence-based screening method for heterologous expression of biosynthetic pathways in fungi. Hoefgen S, Lin J, Fricke J, Stroe MC, Mattern DJ, Kufs JE, Hortschansky P, Brakhage AA, Hoffmeister D, Valiante V. Metab Eng. 2018 Jul;48:44-51. doi: 10.1016/j.ymben.2018.05.014. Epub 2018 May 26. 10.1016/j.ymben.2018.05.014 PubMed 29842926
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