pV2A-T
(Plasmid
#153022)
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Purpose(Empty Backbone) Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153022 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepVV.01
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Backbone manufacturerVito Valiante
- Backbone size (bp) 1820
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Modifications to backboneInsertion of a split YFP, single EcoRV cloning site, TetON promoter systhem, and a TrpC terminator
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Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pV2A-T was a gift from Vito Valiante (Addgene plasmid # 153022 ; http://n2t.net/addgene:153022 ; RRID:Addgene_153022) -
For your References section:
Facile assembly and fluorescence-based screening method for heterologous expression of biosynthetic pathways in fungi. Hoefgen S, Lin J, Fricke J, Stroe MC, Mattern DJ, Kufs JE, Hortschansky P, Brakhage AA, Hoffmeister D, Valiante V. Metab Eng. 2018 Jul;48:44-51. doi: 10.1016/j.ymben.2018.05.014. Epub 2018 May 26. 10.1016/j.ymben.2018.05.014 PubMed 29842926
