pMGE-T7
(Plasmid
#153021)
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Purpose(Empty Backbone) Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153021 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepVV.01
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Backbone manufacturerVito Valiante
- Backbone size (bp) 1820
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Modifications to backboneInsertion of T7 promoters, LacO sequences, and terminators
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Vector typeBacterial Expression
- Promoter T7
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMGE-T7 was a gift from Vito Valiante (Addgene plasmid # 153021 ; http://n2t.net/addgene:153021 ; RRID:Addgene_153021) -
For your References section:
Rational design of flavonoid production routes using combinatorial and precursor-directed biosynthesis. Kufs JE, Hoefgen S, Rautschek J, Bissell AU, Graf C, Fiedler J, Braga D, Regestein L, Rosenbaum MA, Thiele J, Valiante V. ACS Synth Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00172. 10.1021/acssynbio.0c00172 PubMed 32525654
